In-solution staining and arraying method for the immunofluorescence detection of γH2AX foci optimized for clinical applications
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چکیده
منابع مشابه
In-solution staining and arraying method for the immunofluorescence detection of γH2AX foci optimized for clinical applications.
Immunofluorescence quantification of γH2AX foci is a powerful approach to quantify DNA double-strand breaks induced by cancer therapy or accidental exposure to ionizing radiation. Here we report a modification to the γH2AX immunofluorescence labeling method, whereby cells are stained in-solution before being spotted and fixed onto microscope slides. Our modified method allows arraying of 16 pat...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 2011
ISSN: 0736-6205,1940-9818
DOI: 10.2144/000113738